Introduction to the steps and reagents involved in tissue culture, at the Sefton Lab, Salk Institute, San Diego, California.

Provides a brief list of links of importance to the cell culturist. General methods for culture of cell and tissue lines. Links to descriptions of specific cell lines and websites of suppliers. Maintained by the University of New South Wales School of Anatomy.

Collection of protocols spanning the field, and link to forum for methodology discussion.

Describes the equipment and work space requirements for cell and tissue culture. Also details cryopreservation, maintenance and media prep procedures. Important safety considerations are addressed.